Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13559

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1656

Format

Azide and BSA Free
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gelsolin/GSN. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:500.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Gelsolin/GSN antibody [GT1656] detects Gelsolin/GSN protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000.
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Gelsolin/GSN antibody [GT1656] detects secreted Gelsolin/GSN protein in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free and earn rewards!

Have you used Gelsolin/GSN Antibody (GT1656) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies