Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25480

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1930 expressed in HEK293

Format

Azide and BSA Free
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Gelsolin/GSN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Western Blot: Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free [NBP3-25480] -

Western Blot: Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free [NBP3-25480] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1930] (NBP3-25480) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Western Blot: Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free [NBP3-25480] -

Western Blot: Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free [NBP3-25480] - Mouse plasma and rat plasma (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1930] (NBP3-25480) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free [NBP3-25480] -

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free [NBP3-25480] - Gelsolin antibody [HL1930] detects Gelsolin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Gelsolin stained by Gelsolin antibody [HL1930] (NBP3-25480) diluted at 1:500. Blue: Fluoroshield with DAPI.
Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Western Blot: Gelsolin/GSN Antibody (HL1930) [NBP3-25480] -

Non-transfected (-) and transfected (+) HeLa whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1930] (NBP3-25480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Gelsolin/GSN Antibody (HL1930) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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