Geminin Antibody (8H8) - BSA Free

Novus Biologicals | Catalog # NBP3-15158

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8H8 expressed in HEK293

Format

BSA Free
View all Geminin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Geminin. UniProt ID (O75496).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Geminin Antibody (8H8) - BSA Free

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (8H8) [NBP3-15158]

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (8H8) [NBP3-15158]

Immunocytochemistry/Immunofluorescence: Geminin Antibody (8H8) [NBP3-15158] - Immunofluorescence staining of Hela Cells with at Geminin antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Geminin Antibody (8H8) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Geminin

Geminin is a multifunctional nuclear protein invloved in embyro development/differentiation, cell cycle regulation and DNA replciation. Originally defined as an inhibitor of DNA replication and substrate of the anaphase promoting complex, Geminin has also been shown to expand the neural plate in the developing embryos.

Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.

Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Gene Symbol

GMNN

Additional Geminin Products

Product Documents for Geminin Antibody (8H8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Geminin Antibody (8H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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