Geminin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-40826

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Geminin Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Geminin Antibody - BSA Free

Immunohistochemistry: Geminin Antibody [NB100-40826]

Immunohistochemistry: Geminin Antibody [NB100-40826]

Immunohistochemistry: Geminin Antibody [NB100-40826] - Detection of human Geminin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin (NB100-40826). Detection: DAB
Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826]

Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826]

Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Geminin used at a dilution of 1:250.
Immunohistochemistry: Geminin Antibody [NB100-40826]

Immunohistochemistry: Geminin Antibody [NB100-40826]

Immunohistochemistry: Geminin Antibody [NB100-40826] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Immunoprecipitation: Geminin Antibody [NB100-40826]

Immunoprecipitation: Geminin Antibody [NB100-40826]

Immunoprecipitation: Geminin Antibody [NB100-40826] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 mcg/mg lysate. Geminin was also immunoprecipitated by another rabbit anti-Geminin antibodies and NB100-40827, which recognize downstream epitopes.
Immunoprecipitation: Geminin Antibody [NB100-40826]

Immunoprecipitation: Geminin Antibody [NB100-40826]

Immunoprecipitation: Geminin Antibody [NB100-40826] - Detection of human Geminin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 ug per reaction. Geminin was also immunoprecipitated by rabbit anti-Geminin antibody NB100-40827. For blotting immunoprecipitated Geminin, NB100-40826 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Geminin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Geminin

Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex. It is degraded during the mitotic phase of the cell cycle, has a destruction box sequence (RRTLKVIQP), and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Further, geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Entrez Gene IDs

51053 (Human)

Gene Symbol

GMNN

UniProt

NP_056979.1 (Human)

Additional Geminin Products

Product Documents for Geminin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Geminin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Geminin Antibody - BSA Free

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Protocols

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FAQs

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