GFAP Antibody (OTI4D11)

Novus Biologicals | Catalog # NBP1-47782

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4D11
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

This GFAP Antibody (OTI4D11) was developed against full-length protein expressed in 293T cell transfected with human GFAP expression vector.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Astrocyte Marker

Specificity

This antibody is specific for Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

49.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GFAP Antibody (OTI4D11)

Western Blot: GFAP Antibody (OTI4D11) [NBP1-47782]

Western Blot: GFAP Antibody (OTI4D11) [NBP1-47782]

Western Blot: GFAP Antibody (OTI4D11) [NBP1-47782] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded thyroid tissue using anti-GFAP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-GFAP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded bladder tissue using anti-GFAP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded Kidney tissue using anti-GFAP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded lung tissue using anti-GFAP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded lymph node tissue using anti-GFAP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782]

Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded prostate tissue using anti-GFAPmouse monoclonal antibody.

Applications for GFAP Antibody (OTI4D11)

Application
Recommended Usage

Immunohistochemistry

1:50-1:150

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GFAP

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human)

Gene Symbol

GFAP

Additional GFAP Products

Product Documents for GFAP Antibody (OTI4D11)

Certificate of Analysis

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Product Specific Notices for GFAP Antibody (OTI4D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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