GFER/ALR Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03816

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GFER/ALR Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 81-205 of human GFER/ALR (NP_005253.3). MRTQQKRDTKFREDCPPDREELGRHSWAVLHTLAAYYPDLPTPEQQQDMAQFIHLFSKFYPCEECAEDLRKRLCRNHPDTRTRACFTQWLCHLHNEVNRKLGKPDFDCSKVDERWRDGWKDGSCD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GFER/ALR Antibody - BSA Free

GFER/ALR Antibody - BSA Free

Western Blot: GFER/ALR Antibody - BSA Free [NBP3-03816] -

Western Blot: GFER/ALR Antibody - BSA Free [NBP3-03816] - Western blot analysis of various lysates, using GFER/ALR Rabbit pAb (A5463) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
GFER/ALR Antibody - BSA Free

Immunohistochemistry: GFER/ALR Antibody - BSA Free [NBP3-03816] -

Immunohistochemistry: GFER/ALR Antibody - BSA Free [NBP3-03816] - Immunohistochemistry analysis of GFER/ALR in paraffin-embedded Mouse brain tissue using GFER/ALR Rabbit pAb (A5463) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
GFER/ALR Antibody - BSA Free

Immunohistochemistry: GFER/ALR Antibody - BSA Free [NBP3-03816] -

Immunohistochemistry: GFER/ALR Antibody - BSA Free [NBP3-03816] - Immunohistochemistry analysis of GFER/ALR in paraffin-embedded Rat brain tissue using GFER/ALR Rabbit pAb (A5463) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
GFER/ALR Antibody - BSA Free

Immunohistochemistry: GFER/ALR Antibody - BSA Free [NBP3-03816] -

Immunohistochemistry: GFER/ALR Antibody - BSA Free [NBP3-03816] - Immunohistochemistry analysis of GFER/ALR in paraffin-embedded Mouse spleen tissue using GFER/ALR Rabbit pAb (A5463) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for GFER/ALR Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/m/L

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GFER/ALR

The hepatotrophic factor designated augmenter of liver regeneration (ALR) is thought to be one of the factorsresponsible for the extraordinary regenerative capacity of mammalian liver. It has also been called hepaticregenerative stimulation substance (HSS). The gene resides on chromosome 16 in the interval containing the locus forpolycystic kidney disease (PKD1). The putative gene product is 42% similar to the scERV1 protein of yeast. The yeastscERV1 gene had been found to be essential for oxidative phosphorylation, the maintenance of mitochondrial genomes,and the cell division cycle. The human gene is both the structural and functional homolog of the yeast scERV1 gene.(provided by RefSeq)

Long Name

Growth Factor, Augmenter of Liver Regeneration

Alternate Names

ALR, ERV1, Hepatopoietin, HERV1, HPO

Gene Symbol

GFER

Additional GFER/ALR Products

Product Documents for GFER/ALR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GFER/ALR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

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Protocols

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