GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89774

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GFR alpha-3/GDNF R alpha-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPTESRLMNSCLQARRKCQADPTCSAAYHHLDSCTSSISTPLPSEEPSVPADCLEAAQQLRNSSLIGCMCHRRMKNQVACL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunocytochemistry/ Immunofluorescence: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunocytochemistry/Immunofluorescence: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774]

Immunohistochemistry-Paraffin: GFR alpha-3/GDNF R alpha-3 Antibody [NBP1-89774] - Staining of human lung shows strong membranous positivity in pneumocytes.
GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free Western Blot: GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free [NBP1-89774]

Western Blot: GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free [NBP1-89774]

Analysis in control (vector only transfected HEK293T lysate) and GFRA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFR alpha-3/GDNF R alpha-3

Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN), play key roles in the control of vertebrate neuronal survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphatidylinositol linked protein GFRalpha and Ret receptor tyrosine kinase GFRbeta. Following the identification of GFRalpha-1 and 2, another receptor in the GFR family was identified in human and mouse and designated GFRalpha-3. GFRalpha-3 binds persephin. Thus, persephin, GFRalpha-3, and Ret PTK form a complex to transmit the persephin signal and to mediate persephin function.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 3

Alternate Names

GDNF R alpha-3, GFR alpha3, GFRa-3, GFRA3

Entrez Gene IDs

2676 (Human)

Gene Symbol

GFRA3

UniProt

O60609

Additional GFR alpha-3/GDNF R alpha-3 Products

Product Documents for GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GFR alpha-3/GDNF R alpha-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family

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Protocols

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FAQs

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