GGA3 Antibody

Novus Biologicals | Catalog # NB100-2444

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat, Canine

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GGA3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EQLSTEVGEVDQ corresponding to C-Terminus according to NP_619525.1, NP_054720.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GGA3 Antibody

Immunohistochemistry-Paraffin: GGA3 Antibody [NB100-2444]

Immunohistochemistry-Paraffin: GGA3 Antibody [NB100-2444]

Immunohistochemistry-Paraffin: GGA3 Antibody [NB100-2444] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GGA3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 65 kDa band in mouse and rat brain lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.0 kDa band according to NP_766636.2 and 70.2 kDa band according to NP_001238996.1. The 65 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GGA3

GGA3 encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network (TGN) and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma- adaptins. GGA3 mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Alternative splicing of this gene results in two transcript variants.

Alternate Names

ADP-ribosylation factor-binding protein GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, golgi-associated, gamma adaptin ear containing, ARF binding protein 3, KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3

Entrez Gene IDs

23163 (Human)

Gene Symbol

GGA3

UniProt

NP_619525.1 (Human)

Additional GGA3 Products

Product Documents for GGA3 Antibody

Certificate of Analysis

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Product Specific Notices for GGA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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