Gigaxonin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-49924

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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Gigaxonin Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 150 and 200 of human Gigaxonin using the numbering given in entry NP_071324.1 (GeneID 8139).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gigaxonin Antibody - BSA Free

Western Blot: Gigaxonin Antibody [NBP1-49924]

Western Blot: Gigaxonin Antibody [NBP1-49924]

Gigaxonin-Antibody-Western-Blot-NBP1-49924-img0002.jpg
Immunoprecipitation: Gigaxonin Antibody [NBP1-49924]

Immunoprecipitation: Gigaxonin Antibody [NBP1-49924]

Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49924 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49923, which recognizes an upstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: Gigaxonin Antibody [NBP1-49924]

Immunoprecipitation: Gigaxonin Antibody [NBP1-49924]

Gigaxonin-Antibody-Immunoprecipitation-NBP1-49924-img0003.jpg
Gigaxonin Antibody

Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - U251 cells after 48hrs.

Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - Transfection with wild type Gigaxonin. Image from verified customer review.
Gigaxonin Antibody

Western Blot: Gigaxonin Antibody [NBP1-49924] -

Western Blot: Gigaxonin Antibody [NBP1-49924] - Design of proteomic bait to determine the binding partners of GIG.(A) GIG is a BTB/Kelch protein, & these domains are separated by an intervening BACK domain. GIG is thought to dimerize through its BTB domain [16]. The BTB domain has also been shown to bind to E3 ligases, potentially for ubiquitin tagging of Kelch targets. To identify potential substrates for degradation/modification (Kelch binding partners) we expressed flag-tagged full-length GIG (B) or its BTB domain (C). (D) Sequence of proteomic baits with key regions identified by color. The constructs expressed included a single flag tag & linker (grey), Biotag (black), BTB domain (blue), BACK domain (red), & Kelch domains (green). The location of the truncation for the BTB bait shown in (C) is indicated by the black line at amino acid 268, towards the N-terminus of the BACK domain. (E) Flag-tagged constructs were expressed in HEK293 cells & affinity purified with an anti-Flag antibody (abbreviated as F). The membrane was then probed with anti-Flag tag antibodies. Untransfected cells (NC) were included to control for non-specific binding to the antibody or beads. Flag-tagged GIG was ~62 kDa, while the BTB domain was ~34 kDa & forms a dimer in the immunoprecipitation sample that was ~70 kDa. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26460568), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Gigaxonin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Valdiated from a verified customer review

Immunoprecipitation

2-5 ug/mg lysate

Reviewed Applications

Read 1 review rated 4 using NBP1-49924 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Gigaxonin

This gene encodes a member of the cytoskeletal BTB/kelch (Broad-Complex, Tramtrack and Bric a brac) repeat family. (Kelch repeats are predicted to form a beta-propeller shape.) The encoded protein plays a role in neurofilament architecture and is mutated in giant axonal neuropathy.

Alternate Names

FLJ38059, GAN1KLHL16Kelch-like protein 16, giant axonal neuropathy (gigaxonin), gigaxonin

Entrez Gene IDs

8139 (Human)

Gene Symbol

GAN

UniProt

Q9H2C0 (Human)

Additional Gigaxonin Products

Product Documents for Gigaxonin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Gigaxonin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Gigaxonin Antibody - BSA Free

Customer Reviews for Gigaxonin Antibody - BSA Free (1)

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  • Gigaxonin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: U251 glioma cell line
    Species: Human
    Verified Customer | Posted 06/14/2023
    U251 cells after 48hrs. transfection with wild type gigaxonin
    Gigaxonin Antibody - BSA Free NBP1-49924
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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