GKAP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84669

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GKAP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQALVLKDG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GKAP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GKAP1 Antibody [NBP1-84669]

Immunocytochemistry/ Immunofluorescence: GKAP1 Antibody [NBP1-84669]

Immunocytochemistry/Immunofluorescence: GKAP1 Antibody [NBP1-84669] - Staining of human cell line U-2 OS shows localization to cytosol & microtubule organizing center.
Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669] - Staining in human testis and pancreas tissues using anti-GKAP1 antibody. Corresponding GKAP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669] - Staining of human cerebral cortex, kidney, pancreas and testis using Anti-GKAP1 antibody NBP1-84669 (A) shows similar protein distribution across tissues to independent antibody NBP2-62650 (B).
Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669]

Immunohistochemistry-Paraffin: GKAP1 Antibody [NBP1-84669] - Staining of human kidney.
GKAP1 Antibody - BSA Free Western Blot: GKAP1 Antibody - BSA Free [NBP1-84669]

Western Blot: GKAP1 Antibody - BSA Free [NBP1-84669]

Analysis in control (vector only transfected HEK293T lysate) and GKAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410835).

Applications for GKAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GKAP1

The GKAP1 gene encodes a G kinase-anchoring protein 1 that is thought to be involved in germ cell development as it localizes with the Golgi and recruits cGMP-dependent protein kinase I alpha to the Golgi in the mouse tests. It exists in two isoforms with isoform 1 at a length of 366 amino acids at 42 kDA and isoform 2 is 315 amino acids long at 36 kDa. The GKAP1 gene has been known to interact with other genes FXR2, HBP1, HGS, PRKG1, and UBQLN4. It has been researched regarding its role in different diseases such as leukemia, ataxia, and pancreatitis.

Alternate Names

cGMP-dependent protein kinase-anchoring protein of 42 kDa, FKSG21, FLJ25469, G kinase anchoring protein 1, G kinase-anchoring protein 1, GKAP42cGMP-dependent protein kinase anchoring protein 42kDa, protein kinase anchoring protein GKAP42

Gene Symbol

GKAP1

Additional GKAP1 Products

Product Documents for GKAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GKAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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