GLDC Antibody

Novus Biologicals | Catalog # NBP1-32907

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GLDC Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLDC. The exact sequence is proprietary.

Reactivity Notes

Chicken (85%), Xenopus laevis (86%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLDC Antibody

Immunocytochemistry/ Immunofluorescence: GLDC Antibody [NBP1-32907]

Immunocytochemistry/ Immunofluorescence: GLDC Antibody [NBP1-32907]

Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP1-32907] - Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500
Immunoprecipitation: GLDC Antibody [NBP1-32907]

Immunoprecipitation: GLDC Antibody [NBP1-32907]

Immunoprecipitation: GLDC Antibody [NBP1-32907] - Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ug of Glycine dehydrogenase antibody [N3C2-2], Internal. Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal. EasyBlot anti-Rabbit IgG(was used as a secondary reagent.
GLDC Antibody

Western Blot: GLDC Antibody [NBP1-32907] -

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (NBP1-32907) diluted at 1:1000 and competitor's antibody (CST#12794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GLDC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Simple Western

1:200

Western Blot

1:500-1:3000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 111 kDa.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GLDC

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase),H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (alipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes;see MIM 238310, MIM 238330, and MIM 238331.(supplied by OMIM)

Alternate Names

EC 1.4.4.2, GCE, GCSPMGC138198, Glycine cleavage system P protein, glycine cleavage system protein P, Glycine decarboxylase, glycine decarboxylase P-protein, glycine dehydrogenase (decarboxylating), glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P), glycine dehydrogenase [decarboxylating], mitochondrial, HYGN1, MGC138200, NKH

Gene Symbol

GLDC

Additional GLDC Products

Product Documents for GLDC Antibody

Certificate of Analysis

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Product Specific Notices for GLDC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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