GLDC Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83374

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLDC Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ILNANYMAKRLETHYRILFRGARGYVGHEFILDTRPFKKSANIEAVDVAKRLQDYGFHAPTMSWPVAGTLMVEPTESEDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSHWDRPYSREVAAFPLPFVKPENK

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25855294).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLDC Antibody - BSA Free

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Analysis in human placenta and skeletal muscle tissues. Corresponding GLDC RNA-seq data are presented for the same tissues.
Western Blot: GLDC Antibody [NBP1-83374]

Western Blot: GLDC Antibody [NBP1-83374]

Western Blot: GLDC Antibody [NBP1-83374] - Western blot analysis in human cell lines Caco-2 and HeLa. Corresponding GLDC RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Western Blot: GLDC Antibody [NBP1-83374]

Western Blot: GLDC Antibody [NBP1-83374]

Western Blot: GLDC Antibody [NBP1-83374] - Western blot analysis in mouse liver tissue and rat liver tissue.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human kidney shows moderate granular positivity in cytoplasm in cells in tubules.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human liver shows strong granular positivity in cytoplasm in hepatocytes.
GLDC Antibody - BSA Free Western Blot: GLDC Antibody - BSA Free [NBP1-83374]

Western Blot: GLDC Antibody - BSA Free [NBP1-83374]

Analysis in Caco-2 cells) transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLDC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for GLDC Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25855294). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLDC

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase),H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (alipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes;see MIM 238310, MIM 238330, and MIM 238331.(supplied by OMIM)

Alternate Names

EC 1.4.4.2, GCE, GCSPMGC138198, Glycine cleavage system P protein, glycine cleavage system protein P, Glycine decarboxylase, glycine decarboxylase P-protein, glycine dehydrogenase (decarboxylating), glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P), glycine dehydrogenase [decarboxylating], mitochondrial, HYGN1, MGC138200, NKH

Gene Symbol

GLDC

Additional GLDC Products

Product Documents for GLDC Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLDC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLDC Antibody - BSA Free

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Protocols

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