Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3A9
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Product Specifications
Immunogen
Full length human recombinant protein of human GLI4 (NP_612474) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GLI4 Antibody (OTI3A9)
![Western Blot: GLI4 Antibody (OTI3A9) [NBP2-45874]](https://resources.rndsystems.com/images/products/GLI4-Antibody-3A9-Western-Blot-NBP2-45874-img0003.jpg "Western Blot: GLI4 Antibody (OTI3A9) [NBP2-45874]")
Western Blot: GLI4 Antibody (OTI3A9) [NBP2-45874]
Western Blot: GLI4 Antibody (3A9) [NBP2-45874] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI4.![Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874]](https://resources.rndsystems.com/images/products/GLI4-Antibody-3A9-Immunohistochemistry-NBP2-45874-img0002.jpg "Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874]")
Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874]
Immunohistochemistry: GLI4 Antibody (3A9) [NBP2-45874] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874]](https://resources.rndsystems.com/images/products/GLI4-Antibody-3A9-Immunohistochemistry-NBP2-45874-img0001.jpg "Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874]")
Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874]
Immunohistochemistry: GLI4 Antibody (OTI3A9) [NBP2-45874] - GLI4 Antibody (3A9) [NBP2-45874] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for GLI4 Antibody (OTI3A9)
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GLI4
Alternate Names
GLI family zinc finger 4, GLI-Kruppel family member GLI4, glioma-associated oncogene family zinc finger 4, HKR4GLI-Kruppel family member GLI4 (oncogene HKR4), Krueppel-related zinc finger protein 4, Protein HKR4, zinc finger protein GLI4, ZNF928
Gene Symbol
GLI4
Additional GLI4 Products
Product Documents for GLI4 Antibody (OTI3A9)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GLI4 Antibody (OTI3A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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