Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Glu-Glu Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Goats were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polymavirus conjugated to KLH.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glu-Glu Epitope Tag Antibody - BSA Free

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-353]

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-353]

Western Blot: Glu-Glu Epitope Tag Antibody [NB600-353] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-Glu-Glu antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-353]

Western Blot: Glu-Glu Epitope Tag AntibodyBSA Free [NB600-353]

Western Blot: Glu-Glu Epitope Tag Antibody [NB600-353] - Analysis using the HRP conjugate of NB600-353. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for Glu-Glu Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Western Blot

1:1000 - 1:30000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Glu-Glu Epitope Tag

The Glu-Glu epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized Glu-Glu epitope represents amino acid residues 314-319, EYMPME, of middle T antigen of mouse polyomavirus.

Alternate Names

EYMPME epitope tag, EYMPME tag, Glu-Glu epitope tag

Additional Glu-Glu Epitope Tag Products

Product Documents for Glu-Glu Epitope Tag Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glu-Glu Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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