Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Novus Biologicals | Catalog # NBP2-22125

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Simple Western

Cited:

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H7

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human Glucose 6 Phosphate Dehydrogenase expressed in E. coli. [Uniprot: P11413]

Marker

Cytosol Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7)BSA Free [NBP2-22125]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7)BSA Free [NBP2-22125]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded kidney cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.
Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of Jurkat cells using Glucose 6 Phosphate Dehydrogenase mouse mAb (green) and negative control (red).
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7)BSA Free [NBP2-22125]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7)BSA Free [NBP2-22125]

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mAb against human Glucose 6 Phosphate Dehydrogenase (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)
Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded breast cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.
ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125]

ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).
Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Detection of Glucose 6 Phosphate Dehydrogenase in A431 Human Cell Line by Flow Cytometry.

A431 human skin carcinoma cell line was stained with Mouse anti-Glucose 6 Phosphate Dehydrogenase Protein-G purified Monoclonal Antibody conjugated to Phycoerythrin (Catalog # NBP2-22125PE, blue histogram) or matched control antibody (Catalog # NBP2-27231PE, orange histogram).
Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Simple Western: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125] -

Simple Western: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125] - B[a]P-Induced Changes in Cell Cycle. (a) Beas-2B cells treated with 1 µM or 10 µM B[a]P (24 h), 3 µg/mL aphidicolin (18 h), & 100 ng/mL nocodazole (18 h) were prepared for cell imaging post-treatment & immunofluorescent staining. A reference cell cycle diagram is shown. The categorization schematic applied to immunofluorescent images is presented as well as a reference diagram of mammalian cell cycle. Scale bar = 25 μm. (b) Beas-2B cells treated with B[a]P were assessed for WST1 metabolism. n = 5. (c) Quantitative EdU uptake among EdU + Beas-2B cells treated with B[a]P. n = 4. (d) Cumulative cell cycle phase distributions of Beas-2B cells treated with B[a]P or cell cycle controls are presented. n = 4, except nocodazole-treated Beas-2B for which n = 2. (e) Magnitude changes compared to untreated controls among each categorical cell cycle phase are presented; bar colors coincide with phase designations presented in (d). n = 4, except nocodazole-treated Beas-2B for which n = 2. Positive changes denote increased percentage of cells in a specific phase among treated cells, whilst negative values denote decreased percentage. Endpoints are the arithmetic mean of listed independent experiments with error bars denoting one standard error of the mean. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37217524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:50

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 4 using NBP2-22125 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G6PD

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Glucose-6-Phosphate Dehydrogenase

Alternate Names

G6PDH

Entrez Gene IDs

2539 (Human)

Gene Symbol

G6PD

UniProt

Additional G6PD Products

Product Documents for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free

Customer Reviews for Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell
    Species: Human
    Verified Customer | Posted 09/08/2015

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