GluR2 Antibody (9R1X2)

Novus Biologicals | Catalog # NBP3-15370

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9R1X2 expressed in HEK293
View all GluR2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human GluR2 (P42262). YLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GluR2 Antibody (9R1X2)

Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370]

Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370]

Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunocytochemistry/Immunofluorescence: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of SH-SY5Y cells using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370]

Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GluR2 Antibody (9R1X2)

Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] -

Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Rat brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
GluR2 Antibody (9R1X2)

Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] -

Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for GluR2 Antibody (9R1X2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GluR2

The GRIA2 gene encodes for glutamate receptor 2 with an isoform flop of 883 amino acids, 98 kDA, isoform flip of 883 amino acids and 98 kDA, and an isoform 3 of 901 amino acids long and 100 kDA. Glutamate receptors function as a ligand-gated ion channel in the central nervous system and are the predominant excitatory neurotransmitter receptors in the brain. GRIA2 has been researched regarding its role in Alzheimer's disease, multiple sclerosis, motor neuron disease, epilepsy, anorexia, bioplar disorder, and neurodegeneration. GRIA2 is involved in the CREB pathway, intracellular calcium signaling, glutamic acid signaling, and calcium channels as well as interacts with genes NSF, GRIA3, DLG4, GRID2, and SDCBP.

Long Name

Glutamate Receptor 2

Alternate Names

AMPA2, GluA2, GluR-K2, GLURB, GRIA2, HBGR2

Gene Symbol

GRIA2

Additional GluR2 Products

Product Documents for GluR2 Antibody (9R1X2)

Certificate of Analysis

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Product Specific Notices for GluR2 Antibody (9R1X2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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