GluR7/GRIK3 Antibody (2B3D1) - BSA Free
Novus Biologicals | Catalog # NBP2-61782
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2B3D1
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human GluR7/GRIK3 (AA: extra 32-173) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GluR7/GRIK3 Antibody (2B3D1) - BSA Free
Western Blot: GluR7/GRIK3 Antibody (2B3D1)BSA Free [NBP2-61782]
Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against human GRIK3 (AA: extra 32-173) recombinant protein. (Expected MW is 42 kDa)Immunocytochemistry/ Immunofluorescence: GluR7/GRIK3 Antibody (2B3D1) - BSA Free [NBP2-61782]
Immunocytochemistry/Immunofluorescence: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis of Hela cells using GRIK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.Western Blot: GluR7/GRIK3 Antibody (2B3D1)BSA Free [NBP2-61782]
Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against HEK293 (1) and GRIK3 (AA: extra 32-173)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/ Immunofluorescence: GluR7/GRIK3 Antibody (2B3D1) - BSA Free [NBP2-61782]
Immunocytochemistry/Immunofluorescence: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis of Hela cells using GRIK3 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.ELISA: GluR7/GRIK3 Antibody (2B3D1) - BSA Free [NBP2-61782]
ELISA: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Applications for GluR7/GRIK3 Antibody (2B3D1) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GluR7/GRIK3
Long Name
Glutamate Receptor 7
Alternate Names
EAA5, GLR7, GluK3, GRIK3
Gene Symbol
GRIK3
Additional GluR7/GRIK3 Products
Product Documents for GluR7/GRIK3 Antibody (2B3D1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GluR7/GRIK3 Antibody (2B3D1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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