Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLUT9 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of mouse GLUT9 (within residues 390-440). [Swiss-Prot# Q7TSK9]

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26626256)

Localization

Membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLUT9 Antibody - BSA Free

Western Blot: GLUT9 Antibody [NBP1-06271]

Western Blot: GLUT9 Antibody [NBP1-06271]

Western Blot: GLUT9 Antibody [NBP1-06271] - Detection of GLUT9 in mouse kidney membrane using NBP1-06271.
Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06271]

Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06271]

Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06271] - IHC-P analysis of a mouse kidney section with GLUT9 antibody at 1:200 dilution. The antibody generated an expected staining with more intensity towards the basolateral membranes of renal tubular epithelial cells.
GLUT9 Antibody - BSA Free

Western Blot: GLUT9 Antibody - BSA Free [NBP1-06271] -

Inhibition of PO-induced hyperuricemia by K-25. LLC-PK1 cells were stimulated with PO (0.25 mM) with or without pretreatment with K-25 (0.5 mg/mL). a After 24 h of incubation, OAT1, OAT3, URAT1, GLUT9, and XO were measured using an immunoblot assay. b LLC-PK1 cells were incubated with K-25 for 24 h, and the intracellular levels of OAT3 and GLUT9 transporters were analyzed using immunofluorescence analysis. Scale bar = 50 μm. c Effect of K-25 on XO inhibition activity. Values are represented as means +/- SEM, *p < 0.05, versus the allopurinol group. The statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) was determined using ANOVA with Bonferroni correction Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30871515), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GLUT9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200

Western Blot

0.5 ug/ml
Application Notes
In Western blot, this antibody generates a band at ~ 55 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLUT9

GLUT9 (glucose transporter 9; SLC2A9) is a multi-pass membrane transporter protein which primarily transport urate and fructose, and may also implicate in urate reabsorption by proximal tubules as well as the transportation of glucose at low rate. GLUT9 belongs to sugar transporter (TC 2.A.1.1) family and SLC2A9 gene encodes two transcripts: a long isoform (GLUT9L/SLC2A9L) and a short isoform (GLUT9S/SLC2A9S). Placenta is one of the few tissues that express both variants suggesting their role in placental hexose transport, and GLUT9 has been shown to transport both glucose and fructose with about 3-fold higher affinity for glucose. GLUT9 has emerged as a high-capacity urate transporter which influences serum uric acid levels; and GLUT9L and GLUT9S isoforms shows strong expression in the basolateral and apical membranes, respectively, of proximal renal tubular cells. GLUT9 is an important modulator responsible for the reabsorption of urate in the apical membrane of the renal proximal tubules, and loss-of-function mutations in GLUT9 gene results in functional impairment associated with renal hypouricemia type 2 (RHUC2), a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Alternate Names

facilitated glucose transporter member 9, GLUT-9, GLUT9Glut9, GLUTX, solute carrier family 2 (facilitated glucose transporter), member 9, UAQTL2, urate voltage-driven efflux transporter 1

Gene Symbol

SLC2A9

Additional GLUT9 Products

Product Documents for GLUT9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLUT9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLUT9 Antibody - BSA Free

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Protocols

View specific protocols for GLUT9 Antibody - BSA Free (NBP1-06271):

GLUT9 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 30 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-GLUT9 primary antibody (NBP1-06271) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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