Glutaminase Antibody (5C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00002744-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5C4

Format

Azide and BSA Free
View all Glutaminase Primary Antibodies »

Product Specifications

Immunogen

GLS (NP_055720, 580 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL

Specificity

GLS - glutaminase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Glutaminase Antibody (5C4) - Azide and BSA Free

Western Blot: Glutaminase Antibody (5C4) [H00002744-M01]

Western Blot: Glutaminase Antibody (5C4) [H00002744-M01]

Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01]

Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01]

Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01] - Analysis of monoclonal antibody to GLS on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: Glutaminase Antibody (5C4) [H00002744-M01]

ELISA: Glutaminase Antibody (5C4) [H00002744-M01]

ELISA: Glutaminase Antibody (5C4) [H00002744-M01] - Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.

Applications for Glutaminase Antibody (5C4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutaminase

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Entrez Gene IDs

2744 (Human)

Gene Symbol

GLS

OMIM

138280 (Human)

UniProt

NP_055720 (Human)

Additional Glutaminase Products

Product Documents for Glutaminase Antibody (5C4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Glutaminase Antibody (5C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutaminase Antibody (5C4) - Azide and BSA Free

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Protocols

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FAQs

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