Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81838

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VPCAMMLELARALDKKLLSLKTVSDSKPDLSLQLIFFDGEEAFLHWSPQDSLYGSRHLAAKMASTPHPPGARGTSQLHGMDLLVLLDLIGAPNPTFPNFFPNSARWFERLQAIEHELHELGLLKDHSLEGRYFQNYSYGGVIQDDH

Reactivity Notes

Rat (84%), Mouse (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Immunohistochemistry: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Immunohistochemistry: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838]

Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human liver shows low expression as expected.
Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] -

Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.
Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] -

Staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.

Applications for Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutaminyl-peptide Cyclotransferase/QPCT

QPCT encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.

Alternate Names

GCT, Glutaminyl cyclase, Glutamyl cyclase

Entrez Gene IDs

25797 (Human)

Gene Symbol

QPCT

UniProt

Additional Glutaminyl-peptide Cyclotransferase/QPCT Products

Product Documents for Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free

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  • Q: Have you tested QPCT Antibody (NBP1-81838) on mice?

    A:

    This antibody has only been tested with human, but the sequence recognized by this antibody shares 85% similarity with mouse. It may cross-react but we can not guarantee it. If you would like to test the antibody in mouse you may be interested in our Innovators Reward Program; through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product.

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