Glutathione Peroxidase 1/GPX1 Antibody

Novus Biologicals | Catalog # NB100-40805

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glutathione Peroxidase 1/GPX1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-REALPAPSDDATA corresponding to internal region according to NP_000572.2.

Specificity

This antibody is expected to recognise isoform 1 ( NP_000572.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 1/GPX1 Antibody

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia
Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805]

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Glutathione Peroxidase 1/GPX1 Antibody

Application
Recommended Usage

Immunohistochemistry

3.7 ug/ml

Immunohistochemistry-Frozen

20 ug/ml

Immunohistochemistry-Paraffin

3.7 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in human liver and kidney lysates (calculated MW of 22.1 kDa band according to NP_000572.2). IHC: Human cerebral cortex shows staining of microglia. Paraffin embedded Human Liver. IHC-Fr: Porcine kidney shows staining of cytoplasm in PCT epithelial cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 1/GPX1

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

CGPx, GPX1, GSHPX1

Entrez Gene IDs

2876 (Human)

Gene Symbol

GPX1

UniProt

NP_000572.2 (Human)

Additional Glutathione Peroxidase 1/GPX1 Products

Product Documents for Glutathione Peroxidase 1/GPX1 Antibody

Certificate of Analysis

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Product Specific Notices for Glutathione Peroxidase 1/GPX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutathione Peroxidase 1/GPX1 Antibody

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Protocols

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