Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16757

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glutathione S-Transferase pi 1/GSTP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione S-Transferase pi 1/GSTP1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757]

Western Blot: GSTP1 Antibody [NBP2-16757] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757]

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757]

Western Blot: GSTP1 Antibody [NBP2-16757] - 50 ug Rat brain lysate/extract 12 % SDS-PAGE GSTP1 antibody dilution: 1:1000.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757] -

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GSTP1 stained by GSTP1 antibody (NBP2-16757) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757] -

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTP1 stained by GSTP1 antibody (NBP2-16757) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16757] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTP1 antibody (NBP2-16757) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq]

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Gene Symbol

GSTP1

UniProt

P09211 (Human)

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Glutathione S-Transferase pi 1/GSTP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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