Glycogen Phosphorylase BB/GPBB Antibody

Novus Biologicals | Catalog # NBP1-32799

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Predicted:

Bovine (91%), Rhesus Macaque (98%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glycogen Phosphorylase BB/GPBB. The exact sequence is proprietary.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30954669). Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%), Chicken (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Glycogen-Phosphorylase-BB-GPBB-Antibody-Western-Blot-NBP1-32799-img0015.jpg
Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - Mouse fore brain. GPBB antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - U87 xenograft. GPBB antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] -

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glycogen Phosphorylase BB/GPBB antibody (NBP1-32799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glycogen Phosphorylase BB/GPBB antibody (NBP1-32799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GPBB antibody (NBP1-32799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPBB antibody (NBP1-32799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Glycogen Phosphorylase BB/GPBB Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glycogen Phosphorylase BB/GPBB

This glycogen phosphorylase is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Alternate Names

PYGB

Entrez Gene IDs

5834 (Human)

Gene Symbol

PYGB

UniProt

Additional Glycogen Phosphorylase BB/GPBB Products

Product Documents for Glycogen Phosphorylase BB/GPBB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glycogen Phosphorylase BB/GPBB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Citations for Glycogen Phosphorylase BB/GPBB Antibody

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