Glycophorin C Antibody (9K8U2)

Novus Biologicals | Catalog # NBP3-15385

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9K8U2 expressed in HEK293
View all Glycophorin C Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 49-128 of human Glycophorin C (P04921). METSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTEFAESADAALQGDPALQDAGDSSRKEYFI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glycophorin C Antibody (9K8U2)

Western Blot: Glycophorin C Antibody (9K8U2) [NBP3-15385]

Western Blot: Glycophorin C Antibody (9K8U2) [NBP3-15385]

Western Blot: Glycophorin C Antibody (9K8U2) [NBP3-15385] - Western blot analysis of extracts of various cell lines, using Glycophorin C (GYPC) Rabbit mAb (NBP3-15385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: Glycophorin C Antibody (9K8U2) [NBP3-15385]

Immunohistochemistry-Paraffin: Glycophorin C Antibody (9K8U2) [NBP3-15385]

Immunohistochemistry-Paraffin: Glycophorin C Antibody (9K8U2) [NBP3-15385] - Immunohistochemistry of paraffin-embedded human placenta using Glycophorin C (GYPC) Rabbit mAb (NBP3-15385) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Glycophorin C Antibody (9K8U2)

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Glycophorin C Antibody (9K8U2)

Western Blot: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Western Blot: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Western blot analysis of various lysates using Glycophorin C Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Glycophorin C Antibody (9K8U2)

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Glycophorin C Antibody (9K8U2)

Western Blot: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Western Blot: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Western blot analysis of lysates from K-562 cells, using Glycophorin C Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Glycophorin C Antibody (9K8U2)

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Glycophorin C Antibody (9K8U2)

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Glycophorin C Antibody (9K8U2)

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] -

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Applications for Glycophorin C Antibody (9K8U2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glycophorin C

Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, butplays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations havebeen described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duchantigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin Cprotein has very little homology with glycophorins A and B. (provided by RefSeq)

Alternate Names

CD236, CD236 antigen, CD236R, Ge, GLPC, Glycoconnectin, glycophorin C (Gerbich blood group), glycophorin-C, Glycophorin-D, Glycoprotein beta, GPCMGC126191, GPD, GYPD, MGC117309, MGC126192, PAS-2, PAS-2', Sialoglycoprotein D

Gene Symbol

GYPC

Additional Glycophorin C Products

Product Documents for Glycophorin C Antibody (9K8U2)

Certificate of Analysis

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Product Specific Notices for Glycophorin C Antibody (9K8U2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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