Glycosyltransferase Activity Kit

Measurement of glycosyltransferase activity
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R&D Systems Glycosyltransferase Activity Assays
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Citations (16)
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Glycosyltransferase Activity Kit Summary

Glycosyltransferase Activity Kit


glycosyltransferases malachite green detection
View Full Assay Principle
The Glycosyltransferase Activity Kit (Catalog # EA001) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all glycosyltransferases that use diphosphonucleotide sugars as donor substrates. This kit utilizes the nucleotidase ENTPD3/CD39L3 as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide diphosphate, such as UDP or GDP, generated during glycosyltransferase reactions.


  • Applicable for all glycosyltransferases that generate leaving groups with a removable phosphate
  • Non-radioactive
  • No time-consuming separation steps (e.g. column chromatography)
  • Provides accurate kinetic analysis of glycosyltransferase reactions
  • Amenable to high-throughput analysis

Kit Contents

  • NS0-expressed Recombinant Human ENTPD3/CD39L3
  • UDP
  • Phosphatase Buffer 1
  • 100 mM MnCl2
  • Phosphate Standard
  • Malachite Green Reagents A & B

Data Example

Activity of B4GalT1

Activity of B4GalT1
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The ability of Recombinant Human beta-1,4-Galactosyltransferase 1/B4GalT1 (Catalog # 3609-GT) to transfer galactose from UDP-galactose to N-acetyl-alpha-D-glucosamine was measured using the Glycosyltransferase Activity Kit (Catalog # EA001). The activity was plotted against enzyme input, and the specific activity of B4GalT1 was determined to be 1806 pmol/min/µg.


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Citations for Glycosyltransferase Activity Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells
    Authors: D Kerselidou, BS Dohai, DR Nelson, S Daakour, N De Cock, ZAO Hassoun, DK Kim, J Olivet, DC El Assal, A Jaiswal, A Alzahmi, D Saha, C Pain, F Matthijsse, P Lemaitre, M Herfs, J Chapuis, B Ghesquiere, D Vertommen, V Kriechbaum, K Knoops, C Lopez-Igle, M van Zandvo, JC Lambert, J Hanson, C Desmet, M Thiry, KJ Lauersen, M Vidal, P Van Vlierb, F Dequiedt, K Salehi-Ash, JC Twizere
    Science Advances, 2021-05-07;7(19):.  2021-05-07
  2. TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2
    Authors: L Zhang, Y Gao, X Zhang, M Guo, J Yang, H Cui, P Kong, X Niu, Y Bi, J Xu, T Yan, Y Ma, J Yang, Y Qian, F Wang, H Li, F Liu, X Cheng, Y Cui
    Theranostics, 2020-09-14;10(24):11339-11358.  2020-09-14
  3. GlycA Levels during the Earliest Stages of Rheumatoid Arthritis: Potential Use as a Biomarker of Subclinical Cardiovascular Disease
    Authors: J Rodríguez-, M Alperi-Lóp, P López, ÁI Pérez-Álva, M Gil-Serret, N Amigó, C Ulloa, L Benavente, FJ Ballina-Ga, A Suárez
    J Clin Med, 2020-08-01;9(8):.  2020-08-01
  4. A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling
    Authors: E Kenney, A Yaparla, JM Hawdon, DM O'Halloran, L Grayfer, I Eleftheria
    Sci Rep, 2020-07-23;10(1):12312.  2020-07-23
  5. Comparative Analysis of High-Throughput Assays of Family-1 Plant Glycosyltransferases
    Authors: K McGraphery, W Schwab
    Int J Mol Sci, 2020-03-23;21(6):.  2020-03-23
  6. Structural basis for dolichylphosphate mannose biosynthesis
    Authors: R Gandini, T Reichenbac, TC Tan, C Divne
    Nat Commun, 2017-07-25;8(1):120.  2017-07-25
  7. A low redox potential affects monoclonal antibody assembly and glycosylation in cell culture
    Authors: B Dionne, N Mishra, M Butler
    J. Biotechnol, 2017-02-11;0(0):.  2017-02-11
  8. Calcium/calmodulin alleviates substrate inhibition in a strawberry UDP-glucosyltransferase involved in fruit anthocyanin biosynthesis.
    Authors: Peng H, Yang T, Whitaker B, Shangguan L, Fang J
    BMC Plant Biol, 2016-09-08;16(1):197.  2016-09-08
  9. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells
    Authors: Brad Dykstra
    Stem Cells, 2016-07-17;0(0):.  2016-07-17
  10. Homozygosity Mapping and Whole-Genome Sequencing Links a Missense Mutation in POMGNT1 to Autosomal Recessive Retinitis Pigmentosa
    Invest Ophthalmol Vis Sci, 2016-07-01;57(8):3601-9.  2016-07-01
  11. Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera
    Sci Rep, 2016-05-05;6(0):25562.  2016-05-05
  12. Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
    Authors: Possner D, Claesson M, Guy J
    PLoS ONE, 2015-08-21;10(8):e0136239.  2015-08-21
  13. Evidence of a novel mevalonate pathway in archaea.
    Authors: Vinokur J, Korman T, Cao Z, Bowie J
    Biochemistry, 2014-06-18;53(25):4161-8.  2014-06-18
  14. Mice overexpressing beta-1,4-Galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis.
    Authors: Vanhooren V, Vandenbroucke R, Dewaele S, Van Hamme E, Haigh J, Hochepied T, Libert C
    PLoS ONE, 2013-12-05;8(12):e79883.  2013-12-05
  15. The structure of Clostridium difficile toxin A glucosyltransferase domain bound to Mn2+ and UDP provides insights into glucosyltransferase activity and product release.
    Oncogene, 2012-07-23;279(17):3085-97.  2012-07-23
  16. Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction.
    PLoS ONE, 2011-08-11;6(8):e23172.  2011-08-11


  1. Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?

    • The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.

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Glycosyltransferase Activity Kit
By Xun Lin on 06/16/2020