Glycosyltransferase Activity Kit Summary
Glycosyltransferase Activity Kit
|View Full Assay Principle|
- Applicable for all glycosyltransferases that generate leaving groups with a removable phosphate
- No time-consuming separation steps (e.g. column chromatography)
- Provides accurate kinetic analysis of glycosyltransferase reactions
- Amenable to high-throughput analysis
- NS0-expressed Recombinant Human ENTPD3/CD39L3
- Phosphatase Buffer 1
- 100 mM MnCl2
- Phosphate Standard
- Malachite Green Reagents A & B
Activity of B4GalT1
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The ability of Recombinant Human beta-1,4-Galactosyltransferase 1/B4GalT1 (Catalog # 3609-GT) to transfer galactose from UDP-galactose to N-acetyl-alpha-D-glucosamine was measured using the Glycosyltransferase Activity Kit (Catalog # EA001). The activity was plotted against enzyme input, and the specific activity of B4GalT1 was determined to be 1806 pmol/min/µg.
Citations for Glycosyltransferase Activity Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells
Authors: D Kerselidou, BS Dohai, DR Nelson, S Daakour, N De Cock, ZAO Hassoun, DK Kim, J Olivet, DC El Assal, A Jaiswal, A Alzahmi, D Saha, C Pain, F Matthijsse, P Lemaitre, M Herfs, J Chapuis, B Ghesquiere, D Vertommen, V Kriechbaum, K Knoops, C Lopez-Igle, M van Zandvo, JC Lambert, J Hanson, C Desmet, M Thiry, KJ Lauersen, M Vidal, P Van Vlierb, F Dequiedt, K Salehi-Ash, JC Twizere
Science Advances, 2021;7(19):. 2021
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2
Authors: L Zhang, Y Gao, X Zhang, M Guo, J Yang, H Cui, P Kong, X Niu, Y Bi, J Xu, T Yan, Y Ma, J Yang, Y Qian, F Wang, H Li, F Liu, X Cheng, Y Cui
Theranostics, 2020;10(24):11339-11358. 2020
A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling
Authors: E Kenney, A Yaparla, JM Hawdon, DM O'Halloran, L Grayfer, I Eleftheria
Sci Rep, 2020;10(1):12312. 2020
GlycA Levels during the Earliest Stages of Rheumatoid Arthritis: Potential Use as a Biomarker of Subclinical Cardiovascular Disease
Authors: J Rodríguez-, M Alperi-Lóp, P López, ÁI Pérez-Álva, M Gil-Serret, N Amigó, C Ulloa, L Benavente, FJ Ballina-Ga, A Suárez
J Clin Med, 2020;9(8):. 2020
Comparative Analysis of High-Throughput Assays of Family-1 Plant Glycosyltransferases
Authors: K McGraphery, W Schwab
Int J Mol Sci, 2020;21(6):. 2020
Structural basis for dolichylphosphate mannose biosynthesis
Authors: R Gandini, T Reichenbac, TC Tan, C Divne
Nat Commun, 2017;8(1):120. 2017
A low redox potential affects monoclonal antibody assembly and glycosylation in cell culture
Authors: B Dionne, N Mishra, M Butler
J. Biotechnol, 2017;0(0):. 2017
Homozygosity Mapping and Whole-Genome Sequencing Links a Missense Mutation in POMGNT1 to Autosomal Recessive Retinitis Pigmentosa
Invest Ophthalmol Vis Sci, 2016;57(8):3601-9. 2016
Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells
Authors: Brad Dykstra
Stem Cells, 2016;0(0):. 2016
Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera
Sci Rep, 2016;6(0):25562. 2016
Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
Authors: Possner D, Claesson M, Guy J
PLoS ONE, 2015;10(8):e0136239. 2015
Mice overexpressing beta-1,4-Galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis.
Authors: Vanhooren V, Vandenbroucke R, Dewaele S, Van Hamme E, Haigh J, Hochepied T, Libert C
PLoS ONE, 2013;8(12):e79883. 2013
The structure of Clostridium difficile toxin A glucosyltransferase domain bound to Mn2+ and UDP provides insights into glucosyltransferase activity and product release.
Oncogene, 2012;279(17):3085-97. 2012
Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction.
PLoS ONE, 2011;6(8):e23172. 2011
Evidence of a novel mevalonate pathway in archaea.
Authors: Vinokur J, Korman T, Cao Z, Bowie J
Biochemistry, 0;53(25):4161-8. 0
Calcium/calmodulin alleviates substrate inhibition in a strawberry UDP-glucosyltransferase involved in fruit anthocyanin biosynthesis.
Authors: Peng H, Yang T, Whitaker B, Shangguan L, Fang J
BMC Plant Biol, 0;16(1):197. 0
Structural basis for dolichylphosphate mannose biosynthesis.
Authors: Gandini R, Reichenbach T, Tan T, Divne C
Nat Commun, 0;8(1):120. 0
Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?
The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.
Phosphatase Activity Assays
Supplemental ELISA Products
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