Glycosyltransferase Activity Kit

Measurement of glycosyltransferase activity
Catalog # Availability Size / Price Qty
EA001
Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (1)

Glycosyltransferase Activity Kit Summary

Glycosyltransferase Activity Kit

 

glycosyltransferases malachite green detection
View Full Assay Principle
The Glycosyltransferase Activity Kit (Catalog # EA001) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all glycosyltransferases that use diphosphonucleotide sugars as donor substrates. This kit utilizes the nucleotidase ENTPD3/CD39L3 as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide diphosphate, such as UDP or GDP, generated during glycosyltransferase reactions.

Features

  • Applicable for all glycosyltransferases that generate leaving groups with a removable phosphate
  • Non-radioactive
  • No time-consuming separation steps (e.g. column chromatography)
  • Provides accurate kinetic analysis of glycosyltransferase reactions
  • Amenable to high-throughput analysis

Kit Contents

  • NS0-expressed Recombinant Human ENTPD3/CD39L3
  • UDP
  • Phosphatase Buffer 1
  • 100 mM MnCl2
  • Phosphate Standard
  • Malachite Green Reagents A & B

Data Example

Activity of B4GalT1

 
Activity of B4GalT1
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The ability of Recombinant Human beta-1,4-Galactosyltransferase 1/B4GalT1 (Catalog # 3609-GT) to transfer galactose from UDP-galactose to N-acetyl-alpha-D-glucosamine was measured using the Glycosyltransferase Activity Kit (Catalog # EA001). The activity was plotted against enzyme input, and the specific activity of B4GalT1 was determined to be 1806 pmol/min/µg.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

Product Datasheets

Citations for Glycosyltransferase Activity Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling
    Authors: E Kenney, A Yaparla, JM Hawdon, DM O'Halloran, L Grayfer, I Eleftheria
    Sci Rep, 2020;10(1):12312.  2020
  2. Comparative Analysis of High-Throughput Assays of Family-1 Plant Glycosyltransferases
    Authors: K McGraphery, W Schwab
    Int J Mol Sci, 2020;21(6):.  2020
  3. GlycA Levels during the Earliest Stages of Rheumatoid Arthritis: Potential Use as a Biomarker of Subclinical Cardiovascular Disease
    Authors: J Rodríguez-, M Alperi-Lóp, P López, ÁI Pérez-Álva, M Gil-Serret, N Amigó, C Ulloa, L Benavente, FJ Ballina-Ga, A Suárez
    J Clin Med, 2020;9(8):.  2020
  4. Structural basis for dolichylphosphate mannose biosynthesis
    Authors: R Gandini, T Reichenbac, TC Tan, C Divne
    Nat Commun, 2017;8(1):120.  2017
  5. A low redox potential affects monoclonal antibody assembly and glycosylation in cell culture
    Authors: B Dionne, N Mishra, M Butler
    J. Biotechnol, 2017;0(0):.  2017
  6. Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera
    Sci Rep, 2016;6(0):25562.  2016
  7. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells
    Authors: Brad Dykstra
    Stem Cells, 2016;0(0):.  2016
  8. Homozygosity Mapping and Whole-Genome Sequencing Links a Missense Mutation in POMGNT1 to Autosomal Recessive Retinitis Pigmentosa
    Invest Ophthalmol Vis Sci, 2016;57(8):3601-9.  2016
  9. Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
    Authors: Possner D, Claesson M, Guy J
    PLoS ONE, 2015;10(8):e0136239.  2015
  10. Mice overexpressing beta-1,4-Galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis.
    Authors: Vanhooren V, Vandenbroucke R, Dewaele S, Van Hamme E, Haigh J, Hochepied T, Libert C
    PLoS ONE, 2013;8(12):e79883.  2013

FAQs

  1. Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?

    • The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.

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Reviews for Glycosyltransferase Activity Kit

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Glycosyltransferase Activity Kit
By Anonymous on 06/16/2020
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