Glypican 3 Antibody (9C2) - BSA Free

Novus Biologicals | Catalog # NBP2-37483

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9C2

Format

BSA Free
View all Glypican 3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Glypican 3 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

65.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glypican 3 Antibody (9C2) - BSA Free

Western Blot: Glypican 3 Antibody (9C2)BSA Free [NBP2-37483]

Western Blot: Glypican 3 Antibody (9C2)BSA Free [NBP2-37483]

Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of breast cancer tissues using GPC3 mouse mAb with DAB staining.
Flow Cytometry: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Flow Cytometry: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Flow Cytometry: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red).
Western Blot: Glypican 3 Antibody (9C2)BSA Free [NBP2-37483]

Western Blot: Glypican 3 Antibody (9C2)BSA Free [NBP2-37483]

Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)
Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of liver cancer tissues using GPC3 mouse mAb with DAB staining.
ELISA: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

ELISA: Glypican 3 Antibody (9C2) - BSA Free [NBP2-37483]

ELISA: Glypican 3 Antibody (9C2) [NBP2-37483] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Glypican 3 Antibody (9C2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol

GPC3

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (9C2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glypican 3 Antibody (9C2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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