GNAL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32225

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (95%), Rat (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GNAL Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAL. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (87%), Xenopus laevis (90%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GNAL Antibody - BSA Free

Western Blot: GNAL Antibody [NBP1-32225]

Western Blot: GNAL Antibody [NBP1-32225]

Western Blot: GNAL Antibody [NBP1-32225] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry: GNAL Antibody [NBP1-32225]

Immunohistochemistry: GNAL Antibody [NBP1-32225]

Immunohistochemistry: GNAL Antibody [NBP1-32225] - Mouse brain tissue. Antibody at 1:500. Selective and strong signal in the striatum. Image submitted by a verified customer review.
Western Blot: GNAL Antibody [NBP1-32225]

Western Blot: GNAL Antibody [NBP1-32225]

Western Blot: GNAL Antibody [NBP1-32225] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-32225]

Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-32225]

Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-32225] - NCI-N87 xenograft. GNAL antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
GNAL Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GNAL Antibody [NBP1-32225] -

GNAL antibody detects GNAL protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAL stained by GNAL antibody (NBP1-32225) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for GNAL Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-32225 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GNAL

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP, and most obviously regulate the activity of the best studied effectors. The Gs subfamily of Galpha subunits includes two closely related proteins, Galpha s and Galpha olf, which, respectively, stimulate adenylate cyclase and mediate response to olfactory stimuli.

Alternate Names

Adenylate cyclase-stimulating G alpha protein, olfactory type, guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory typ, guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory type, guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide, olfactory type, guanine nucleotide-binding protein G(olf) subunit alpha

Gene Symbol

GNAL

Additional GNAL Products

Product Documents for GNAL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GNAL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GNAL Antibody - BSA Free

Customer Reviews for GNAL Antibody - BSA Free (1)

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Customer Images

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  • GNAL Antibody
    Name: Fuming Zhou
    Application: Immunohistochemistry
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 05/14/2018
    Selective and strong immunofluorescent signal in the striatum detected by NBP1-32225.
    immunohistochemistry on brain tissue. NBP1-32225 diluted at 1:500, leading to a strong signal with high selectivity, see the image.
    GNAL Antibody - BSA Free NBP1-32225

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Protocols

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FAQs

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