Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Catalog # Availability Size / Price Qty
F0113
Detection of Rat IgGPrimary Antibody by Flow Cytometry
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Product Details
Citations (2)
FAQs
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, wherethe primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommendedconcentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Rat IgGPrimary Antibody by Flow Cytometry View Larger

Detection of Rat IgGPrimary Antibody by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with RatAnti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994,filled histogram) or isotype control antibody (Catalog # MAB006),open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody
(Catalog # F0113) at 10 μL/106 cells. 

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Deletion of receptor for advanced glycation end products exacerbates lymphoproliferative syndrome and lupus nephritis in B6-MRL Fas lpr/j mice.
    Authors: Goury A, Meghraoui-Kheddar A, Belmokhtar K, Vuiblet V, Ortillon J, Jaisson S, Devy J, Le Naour R, Tabary T, Cohen J, Schmidt A, Rieu P, Toure F
    J Immunol, 2015;194(8):3612-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009;113(24):6120-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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APC-conjugated Antibody

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APC-conjugated Antibody
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APC-conjugated Antibody?

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