Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.
Polyclonal Goat IgG
Rat IgG heavy and light chains
Supplied in a saline solution containing BSA and Sodium Azide.
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, wherethe primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommendedconcentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Rat IgGPrimary Antibody by Flow Cytometry
bEnd.3 mouse endothelioma cell line was stained with RatAnti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994,filled histogram) or isotype control antibody (Catalog # MAB006),open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113) at 10 μL/106 cells.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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