GOLGA5 Antibody (GT2726) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13566

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT2726

Format

Azide and BSA Free
View all GOLGA5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

Reactivity Notes

Mouse (89%), Rat (88%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GOLGA5 Antibody (GT2726) - Azide and BSA Free

Western Blot: GOLGA5 Antibody (GT2726) [NBP3-13566]

Western Blot: GOLGA5 Antibody (GT2726) [NBP3-13566]

Western Blot: GOLGA5 Antibody (GT2726) [NBP3-13566] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLGA5 antibody [GT2726] (NBP3-13566) diluted at 1:1000.
GOLGA5 Antibody (GT2726) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GOLGA5 Antibody (GT2726) [NBP3-13566] -

GOLGA5 antibody [GT2726] detects GOLGA5 protein at Golgi apparatus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLGA5 protein stained by GOLGA5 antibody [GT2726] (NBP3-13566) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for GOLGA5 Antibody (GT2726) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOLGA5

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq]

Alternate Names

Cell proliferation-inducing gene 31 protein, golgi autoantigen, golgin subfamily a, 5, golgin A5, Golgin subfamily A member 5, golgin-84, GOLIM5, Protein Ret-II, PTC5, RET-fused gene 5 protein, RETII, ret-II, rfg5, RFG5golgi integral membrane protein 5

Gene Symbol

GOLGA5

Additional GOLGA5 Products

Product Documents for GOLGA5 Antibody (GT2726) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GOLGA5 Antibody (GT2726) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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