GOLPH4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-91955
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0013.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ENRQLRKAHQDIHTQLQDVKQQHKNLLSEHEQLVVTLEDHKSALAAAQTQVAEYKQLKDTLNRIPSLRKPDPAEQQNVTQVAHSPQGYNTAREKPTREVQEVSRNNDVWQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%).
Marker
Golgi Apparatus Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GOLPH4 Antibody - BSA Free
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0002.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver shows strong cytoplasmic positivity with granular pattern in hepatocytes.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0003.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human skin shows low expression as expected.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0004.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human small intestine shows high expression.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0007.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human cerebral cortex.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0008.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0009.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human cerebral cortex, liver, skin and small intestine using Anti-GOLIM4 antibody NBP1-91955 (A) shows similar protein distribution across tissues to independent antibody NBP1-91954 (B).![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0011.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human small intestine shows strong positivity in Golgi apparatus in glandular cells.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0012.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons and glial cells.![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]](https://resources.rndsystems.com/images/products/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0014.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]")
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955]
Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human skin shows moderate granular cytoplasmic positivity in a small subset of squamous epithelial cells.![GOLPH4 Antibody - BSA Free Immunohistochemistry: GOLPH4 Antibody - BSA Free [NBP1-91955]](https://resources.rndsystems.com/images/products/nbp1-91955_rabbit-polyclonal-golph4-antibody-3052025751586.jpg "Immunohistochemistry: GOLPH4 Antibody - BSA Free [NBP1-91955]")
Applications for GOLPH4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GOLPH4
Alternate Names
GIMPc, GIMPCcis, golgi integral membrane protein 4, Golgi phosphoprotein 4130 kDa golgi-localized phosphoprotein, Golgi phosphoprotein of 130 kDa, GPP130Golgi-localized phosphoprotein of 130 kDa
Gene Symbol
GOLIM4
Additional GOLPH4 Products
Product Documents for GOLPH4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GOLPH4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GOLPH4 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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