gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free
Novus Biologicals | Catalog # NBP1-04346
![Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3)BSA Free [NBP1-04346]](https://resources.rndsystems.com/images/products/gp96-HSP90B1-GRP94-Antibody-2H3-Western-Blot-NBP1-04346-img0007.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3)BSA Free [NBP1-04346]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2H3
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human gp96/HSP90B1/GRP94 (676-803aa) purified from E. coli
Marker
ER Stress Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free
Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3)BSA Free [NBP1-04346]
Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]](https://resources.rndsystems.com/images/products/gp96-HSP90B1-GRP94-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP1-04346-img0006.jpg "Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]")
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]
Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]](https://resources.rndsystems.com/images/products/gp96-HSP90B1-GRP94-Antibody-2H3-Flow-Cytometry-NBP1-04346-img0008.jpg "Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]")
Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free [NBP1-04346]
Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
2-5ug- 1-106cells
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:100-1:200
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: gp96/HSP90B1
Long Name
Heat Shock Protein 90 beta 1
Alternate Names
ECGP, Endoplasmin, Grp94, HSP90B1
Gene Symbol
HSP90B1
UniProt
Additional gp96/HSP90B1 Products
Product Documents for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for gp96/HSP90B1/GRP94 Antibody (2H3) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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