![Western Blot: GPR125 Antibody [NBP1-03561]](https://resources.rndsystems.com/images/products/GPR125-Antibody-Western-Blot-NBP1-03561-img0002.jpg "Western Blot: GPR125 Antibody [NBP1-03561]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence CTNKSASSFKNS corresponding to internal region according to NP_660333.2.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33754041).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GPR125 Antibody
Western Blot: GPR125 Antibody [NBP1-03561]
Western Blot: GPR125 Antibody [NBP1-03561] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]](https://resources.rndsystems.com/images/products/GPR125-Antibody-Immunohistochemistry-Paraffin-NBP1-03561-img0005.jpg "Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]")
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Bouin's fixed Human Testis stained with anti-GPR125. IHC-P image submitted by a verified customer review.![Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]](https://resources.rndsystems.com/images/products/GPR125-Antibody-Immunohistochemistry-Paraffin-NBP1-03561-img0004.jpg "Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]")
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Staining of Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for GPR125 Antibody
Application
Recommended Usage
Immunohistochemistry
3.75 ug/mL
Immunohistochemistry-Paraffin
3.75 ug/mL
Peptide ELISA
Detection limit 1:8000
Western Blot
0.5 - 1.5 ug/mL
Application Notes
WB: Approx. 140 kDa band observed in human ovary lysates (calculated MW of 146 kDa band according to NP_660333.2). An additional band of unknown identity was also consistently observed at 33 kDa. This band was successfully blocked by incubation with the immunizing peptide. GPR125 antibody also validated for IHC-P from a verified customer review.
Reviewed Applications
Read 1 review rated 4 using NBP1-03561 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GPR125
Additional GPR125 Products
Product Documents for GPR125 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPR125 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GPR125 Antibody
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Customer Reviews for GPR125 Antibody (1)
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1 Customer Rating
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Customer Images
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Application: Immunohistochemistry-ParaffinSample Tested: TestisSpecies: HumanVerified Customer | Posted 05/06/2014Bouin's fixed Human Testis stained with anti-GPR125
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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