GPR3 Antibody (3B4-G3) - Azide and BSA Free

Novus Biologicals | Catalog # H00002827-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B4-G3

Format

Azide and BSA Free
View all GPR3 Primary Antibodies »

Product Specifications

Immunogen

GPR3 (AAH32702, 1 a.a. ~ 330 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMWGAGSPLAWLSAGSGNVNVSSVGPAEGPTGPAAPLPSPKAWDVVLCISGTLVSCENALVVAIIVGTPAFRAPMFLLVGSLAVADLLAGLGLVLHFAAVFCIGSAEMSLVLVGVLAMAFTASIGSLLAITVDRYLSLYNALTYYSETTVTRTYVMLALVWGGALGLGLLPVLAWNCLDGLTTCGVVYPLSKNHLVVLAIAFFMVFGIMLQLYAQICRIVCRHAQQIALQRHLLPASHYVATRKGIATLAVVLGAFAACWLPFTVYCLLGDAHSPPLYTYLTLLPATYNSMINPIIYAFRNQDVQKVLWAVCCCCSSSKIPFRSRSPSDV

Specificity

GPR3 - G protein-coupled receptor 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GPR3 Antibody (3B4-G3) - Azide and BSA Free

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01]

Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01]

Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 1 ug/ml.
Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi ( Cat # H00002827-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody (M01), clone 3B4-G3 (Cat # H00002827-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01]

Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - GPR3 monoclonal antibody (M01), clone 3B4-G3 Analysis of GPR3 expression in Jurkat.
Sandwich ELISA: GPR3 Antibody (3B4-G3) [H00002827-M01] - Detection limit for recombinant GST tagged GPR3 is approximately 0.03ng/ml as a capture antibody.

Applications for GPR3 Antibody (3B4-G3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GPR3

GPR3, previously called ACCA (adenylate cyclase constitutive activator), is 7-transmembrane receptor that has been shown to promote cAMP accumulation. In oocytes, cAMP generated by GPR3 regulates maintenance of meiotic arrest. GPR3 is also expressed in the brain, where it has been implicated in cAMP-dependent emotional responses and modulation of amyloid-beta peptide generation.

Long Name

G Protein-coupled Receptor 3

Alternate Names

ACCA, G protein-coupled receptor 3

Entrez Gene IDs

2827 (Human)

Gene Symbol

GPR3

OMIM

600241 (Human)

UniProt

AAH32702 (Human)

Additional GPR3 Products

Product Documents for GPR3 Antibody (3B4-G3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GPR3 Antibody (3B4-G3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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