GRASP55 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35134

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRASP55 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 353-452 of human GRASP55 (NP_056345.3).

Sequence:
PGIAPLPLPSEFLPSFPLVPESSSAASSGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANASESP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRASP55 Antibody - BSA Free

GRASP55 Antibody

Western Blot: GRASP55 Antibody [NBP3-35134] -

Western Blot: GRASP55 Antibody [NBP3-35134] - Western blot analysis of various lysates using GRASP55 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
GRASP55 Antibody

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] -

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] - Immunofluorescence analysis of U-2 OS cells using GRASP55 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.
GRASP55 Antibody

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] -

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] - Immunofluorescence analysis of L929 cells using GRASP55 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.
GRASP55 Antibody

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] -

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP3-35134] - Immunofluorescence analysis of H9c2 cells using GRASP55 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for GRASP55 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRASP55

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus. [provided by RefSeq]

Alternate Names

Golgi phosphoprotein 6, golgi reassembly stacking protein 2, 55 kDa, golgi reassembly stacking protein 2, 55kDa, Golgi reassembly-stacking protein of 55 kDa, GOLPH6DKFZp434D156, GRASP55FLJ13139, GRS2Golgi reassembly-stacking protein 2, p59

Gene Symbol

GORASP2

Additional GRASP55 Products

Product Documents for GRASP55 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRASP55 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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