GRHL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81322

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRHL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VTVSIATMPTHSIKTETQPHGFAVGIPPAVYHPEPTERVVVFDRNLNTDQFSSGAQAPNAQRRTPDSTFSETFKEGVQEVFFPSDLSLRMPGMNSEDYVFDSVSGNNFEYTLEASKSLRQKPGDSTMTYLNK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23690579)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRHL1 Antibody - BSA Free

Western Blot: GRHL1 Antibody [NBP1-81322]

Western Blot: GRHL1 Antibody [NBP1-81322]

Western Blot: GRHL1 Antibody [NBP1-81322] - Analysis in control (vector only transfected HEK293T lysate) and GRHL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: GRHL1 Antibody [NBP1-81322]

Immunocytochemistry/ Immunofluorescence: GRHL1 Antibody [NBP1-81322]

Immunocytochemistry/Immunofluorescence: GRHL1 Antibody [NBP1-81322] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining in human skin and skeletal muscle tissues using NBP1-81322 antibody. Corresponding GRHL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skin shows moderate to strong nuclear positivity in a subset of squamous epithelial cells.
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human esophagus shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human esophagus, placenta, skeletal muscle and skin using Anti-GRHL1 antibody NBP1-81322 (A) shows similar protein distribution across tissues to independent antibody NBP1-81321 (B).
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322]

Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

Applications for GRHL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRHL1

GRHL1 encodes a member of the grainyhead family of transcription factors. The encoded protein interacts with sister of mammalian grainyhead (SOM) and may function as a transcription factor during development. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

grainyhead-like 1 (Drosophila), grainyhead-like protein 1 homolog, LBP protein 32, MGRLBP-32, NH32, TFCP2L2, Transcription factor CP2-like 2LBP32Mammalian grainyhead, Transcription factor LBP-32

Gene Symbol

GRHL1

Additional GRHL1 Products

Product Documents for GRHL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRHL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRHL1 Antibody - BSA Free

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Protocols

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FAQs

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