GRK2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03731

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRK2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2 (NP_001610.2). LGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRK2 Antibody - BSA Free

Western Blot: GRK2 AntibodyBSA Free [NBP3-03731]

Western Blot: GRK2 AntibodyBSA Free [NBP3-03731]

Western Blot: GRK2 Antibody [NBP3-03731] - Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (NBP3-03731) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: GRK2 Antibody - BSA Free [NBP3-03731]

Immunohistochemistry-Paraffin: GRK2 Antibody - BSA Free [NBP3-03731]

Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded mouse kidney using GRK2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry-Paraffin: GRK2 Antibody - BSA Free [NBP3-03731]

Immunohistochemistry-Paraffin: GRK2 Antibody - BSA Free [NBP3-03731]

Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded rat brain using GRK2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry-Paraffin: GRK2 Antibody - BSA Free [NBP3-03731]

Immunohistochemistry-Paraffin: GRK2 Antibody - BSA Free [NBP3-03731]

Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded human esophageal cancer using GRK2 antibody at dilution of 1:200 (40x lens).
Knockout Validated: GRK2 Antibody - BSA Free [NBP3-03731]

Western Blot: GRK2 Antibody - BSA Free [NBP3-03731]

Western Blot: GRK2 Antibody [NBP3-03731] - Analysis of extracts of various cell lines, using GRK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for GRK2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRK2

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Gene Symbol

GRK2

Additional GRK2 Products

Product Documents for GRK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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Protocols

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FAQs

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