![Western Blot: GRK5 AntibodyBSA Free [NBP3-04998]](https://beta-resources.rndsystems.com/images/products/GRK5-Antibody-Western-Blot-NBP3-04998-img0005.jpg "Western Blot: GRK5 AntibodyBSA Free [NBP3-04998]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1). LGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit GRK5 Antibody - BSA Free (NBP3-04998) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GRK5 Antibody - BSA Free
Western Blot: GRK5 AntibodyBSA Free [NBP3-04998]
Western Blot: GRK5 Antibody [NBP3-04998] - Analysis of extracts of various cell lines, using GRK5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit![Immunocytochemistry/ Immunofluorescence: GRK5 Antibody - BSA Free [NBP3-04998]](https://beta-resources.rndsystems.com/images/products/GRK5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04998-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GRK5 Antibody - BSA Free [NBP3-04998]")
Immunocytochemistry/ Immunofluorescence: GRK5 Antibody - BSA Free [NBP3-04998]
Immunocytochemistry/Immunofluorescence: GRK5 Antibody [NBP3-04998] - Analysis of L929 cells using GRK5 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. GRK5 antibody![Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]](https://beta-resources.rndsystems.com/images/products/GRK5-Antibody-Immunohistochemistry-Paraffin-NBP3-04998-img0004.jpg "Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]")
Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]
Immunohistochemistry-Paraffin: GRK5 Antibody [NBP3-04998] - Paraffin-embedded mouse heart using GRK5 antibody at dilution of 1:100 (40x lens).![Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]](https://beta-resources.rndsystems.com/images/products/GRK5-Antibody-Immunohistochemistry-Paraffin-NBP3-04998-img0002.jpg "Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]")
Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]
Immunohistochemistry-Paraffin: GRK5 Antibody [NBP3-04998] - Paraffin-embedded human colon carcinoma using GRK5 antibody at dilution of 1:100 (40x lens).![Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]](https://beta-resources.rndsystems.com/images/products/GRK5-Antibody-Immunohistochemistry-Paraffin-NBP3-04998-img0003.jpg "Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]")
Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]
Immunohistochemistry-Paraffin: GRK5 Antibody [NBP3-04998] - Paraffin-embedded rat heart using GRK5 antibody at dilution of 1:100 (40x lens).Applications for GRK5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GRK5
Long Name
G protein-coupled Receptor Kinase 5
Alternate Names
GPRK5
Gene Symbol
GRK5
Additional GRK5 Products
Product Documents for GRK5 Antibody - BSA Free
Product Specific Notices for GRK5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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