GRP78/HSPA5 Antibody (6H4-2G7) - BSA Free

Novus Biologicals | Catalog # NBP2-12889

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6H4-2G7

Format

BSA Free
View all GRP78/HSPA5 Primary Antibodies »

Product Specifications

Immunogen

His-tagged human GRP78

Localization

Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome

Marker

ER Stress Marker

Specificity

Detects a protein with a mass of approx. 78kDa corresponding to GRP78

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GRP78/HSPA5 Antibody (6H4-2G7) - BSA Free

Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]

Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]

Western Blot: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889] - analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]

Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889]

Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (6H4-2G7) [NBP2-12889] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Applications for GRP78/HSPA5 Antibody (6H4-2G7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml of GRP78 Antibody was sufficient for detection of Grp78 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

0.5 ug/mL was sufficient for detection of Grp78 in 10ug of rat tissue lysate by ECL immunoblot analysis.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody (6H4-2G7) - BSA Free

Certificate of Analysis

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Product Specific Notices for GRP78/HSPA5 Antibody (6H4-2G7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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