GRP78/HSPA5 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56411

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRP78/HSPA5 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 550-600 of human GRP78 was used as the immunogen.

Reactivity Notes

Predicted to react with Chimpanzee, Canine, Macaque, Rat, Xenopus and Zebrafish.

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78/HSPA5 Antibody [NB100-56411]

Western Blot: GRP78/HSPA5 Antibody [NB100-56411]

Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411]

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in formalin-fixed, paraffin-embedded mouse liver tissue using this antibody at 5 ug/ml.
Western Blot: GRP78/HSPA5 Antibody [NB100-56411]

Western Blot: GRP78/HSPA5 Antibody [NB100-56411]

Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in mouse liver tissue enriched using Endoplasmic Reticulum Enrichment Kit and 1 ug/ml of NB100-56411 and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.
Simple Western: GRP78/HSPA5 Antibody [NB100-56411]

Simple Western: GRP78/HSPA5 Antibody [NB100-56411]

Simple Western: GRP78/HSPA5 Antibody [NB100-56411] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Applications for GRP78/HSPA5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Egawa et al (2011))

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:250

Western Blot

1-3 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:250, apparent MW was 77 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

GRP78, a 78 kDa glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycermia as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

UniProt

P11021 (Human)

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRP78/HSPA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRP78/HSPA5 Antibody - BSA Free

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Protocols

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