GSH2 Antibody (HL2533)

Novus Biologicals | Catalog # NBP3-48695

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2533
View all GSH2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human GSH2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSH2 Antibody (HL2533)

GSH2 Antibody (HL2533)

Western Blot: GSH2 Antibody (HL2533) [NBP3-48695] -

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GSH2 Antibody (HL2533)

Western Blot: GSH2 Antibody (HL2533) [NBP3-48695] -

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GSH2 Antibody (HL2533)

Western Blot: GSH2 Antibody (HL2533) [NBP3-48695] -

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GSH2 Antibody (HL2533)

Immunohistochemistry-Paraffin: GSH2 Antibody (HL2533) [NBP3-48695] -

GSH2 antibody [HL2533] detects GSH2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
GSH2 stained by GSH2 antibody [HL2533] (NBP3-48695) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for GSH2 Antibody (HL2533)

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSH2

The GSH2 (also known as GSX2) gene codes for a 304 amino acid long, 32 kDA GS homeobox 2 protein that is a transcription factor that binds to the DNA sequence 5'-CNAATTAG-3'. The GSH2 gene has been researched for its role in leukemia as well as neuronitis. Interactions between GSH2 and ABL1 and NKX2-1 are known to exist.

Alternate Names

GS homeobox 2, GSH2, Homeobox protein GSH-2

Gene Symbol

GSX2

Additional GSH2 Products

Product Documents for GSH2 Antibody (HL2533)

Certificate of Analysis

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Product Specific Notices for GSH2 Antibody (HL2533)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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