GST Epitope Tag Antibody - BSA Free

Novus Biologicals | Catalog # NB600-325

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human, Avian - Chicken, Hamster - Cricetulus (Chinese Hamster)

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all GST Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GST Epitope Tag Antibody - BSA Free

Western Blot: GST Epitope Tag AntibodyBSA Free [NB600-325]

Western Blot: GST Epitope Tag AntibodyBSA Free [NB600-325]

Western Blot: GST Epitope Tag Antibody [NB600-325] - Analysis using the HRP conjugate of NB600-325. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).

Applications for GST Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GST Epitope Tag

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GST Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GST Epitope Tag Antibody - BSA Free

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Protocols

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