Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-GTF2A1-1F2

Format

Azide and BSA Free
View all GTF2A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human GTF2A1 protein (Uniprot: P52655)

Reactivity Notes

Predicted to react in Mouse and Rat.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

41.51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13933).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free

Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054]

Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054]

Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2A1 Antibody (PCRP-GTF2A1-1F2).
GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] -

Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free Flow Cytometry: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] -

Flow Cytometry: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] -

Flow cytometric analysis of PFA-fixed HeLa cells. GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] -

Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free [NBP3-14054] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Applications for GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: GTF2A1

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role intranscriptional activation. TFIIA in a complex with TBP mediates transcriptional activity

Alternate Names

General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, MGC129969, MGC129970, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit

Gene Symbol

GTF2A1

Additional GTF2A1 Products

Product Documents for GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GTF2A1 Antibody (PCRP-GTF2A1-1F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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