GTF3C5 Antibody

Novus Biologicals | Catalog # NB100-60435

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GTF3C5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 469 and 519 of human general transcription factor 3C polypeptide 5 (Transcription factor IIIC 63 kDa subunit) using the numbering given in entry NP_036219.1 (GeneID 9328).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GTF3C5 Antibody

Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435] - Detection of human and mouse GTF3C5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GTF3C5 antibody NB100-60435 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Detection of human GTF3C5/TFIIIC63 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 (NB100-60435). Detection: DAB
Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60435 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry-Paraffin: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry-Paraffin: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry-Paraffin: GTF3C5 Antibody [NB100-60435] - FFPE section of human thyroid carcinoma. Antibody dilution of 1:500.
Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 used at a dilution of 1:500 (0.4ug/ml). Detection: DAB
Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Detection of mouse GTF3C5/TFIIIC63 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 (NB100-60435). Detection: DAB
Immunoprecipitation: GTF3C5 Antibody [NB100-60435]

Immunoprecipitation: GTF3C5 Antibody [NB100-60435]

Immunoprecipitation: GTF3C5 Antibody [NB100-60435] - Detection of human GTF3C5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF3C5 antibody NB100-60435 (lot NB100-60435-2) used for IP at 6 ug per reaction. GTF3C5 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60435-1). For blotting immunoprecipitated GTF3C5, NB100-60435 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for GTF3C5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GTF3C5

General transcription factor 3C polypeptide 5 (GTF3C5/TFIIIC63) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C5/TFIIIC63 interacts with TFIIIB and may facilitate TFIIIB and RNA polymerase III recruitment in association with TFIIIC102, TFIIIB90, and RPC62. Alternate names for GTF3C5/TFIIIC63 include transcription factor IIIC subunit epsilon, TF3C-epsilon, TFIIIC 63 kDa subunit, general transcription factor IIIC polypeptide 5 (63 kDa), and FLJ20857.

Alternate Names

FLJ20857, general transcription factor 3C polypeptide 5, general transcription factor IIIC, polypeptide 5 (63kD), general transcription factor IIIC, polypeptide 5, 63kDa, TF3C-epsilon, TFiiiC2-63, TFIIIC63TFIIIC 63 kDa subunit, TFIIICepsilon, Transcription factor IIIC 63 kDa subunit, Transcription factor IIIC subunit epsilon, transcription factor IIIC, 63 kD

Entrez Gene IDs

9328 (Human); 70239 (Mouse)

Gene Symbol

GTF3C5

UniProt

NP_036219.1 (Human)

Additional GTF3C5 Products

Product Documents for GTF3C5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GTF3C5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GTF3C5 Antibody

There are currently no reviews for this product. Be the first to review GTF3C5 Antibody and earn rewards!

Have you used GTF3C5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies