H1F0 Antibody

Novus Biologicals | Catalog # NBP2-16772

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all H1F0 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human H1F0.0. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for H1F0 Antibody

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP2-16772]

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP2-16772]

Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP2-16772] - Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H1.0 stained by Histone H1.0 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded rat fore brain. Histone H1.0 antibody dilution: 1:500.
H1F0 Antibody

Western Blot: H1F0 Antibody [NBP2-16772] -

Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
H1F0 Antibody

Western Blot: H1F0 Antibody [NBP2-16772] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.0 antibody (NBP2-16772) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
H1F0 Antibody

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] -

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (NBP2-16772) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
H1F0 Antibody

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP2-16772] -

Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H1.0 stained by Histone H1.0 antibody (NBP2-16772) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
H1F0 Antibody

Western Blot: H1F0 Antibody [NBP2-16772] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.0 antibody (NBP2-16772) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
H1F0 Antibody

Western Blot: H1F0 Antibody [NBP2-16772] -

Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for H1F0 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: H1F0

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq]

Alternate Names

H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0

Gene Symbol

H1-0

Additional H1F0 Products

Product Documents for H1F0 Antibody

Certificate of Analysis

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Product Specific Notices for H1F0 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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