HADHA Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03743

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HADHA Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2). IAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HADHA Antibody - BSA Free

HADHA Antibody - BSA Free

Western Blot: HADHA Antibody - BSA Free [HADHA] -

Western Blot: HADHA Antibody - BSA Free [HADHA] - Western blot analysis of lysates from wild type (WT) and HADHA knockout (KO) 293T cells, using [KO Validated] HADHA Rabbit pAb at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western Blot: HADHA AntibodyBSA Free [NBP3-03743]

Western Blot: HADHA AntibodyBSA Free [NBP3-03743]

Western Blot: HADHA Antibody [NBP3-03743] - Analysis of 200ug extracts of HeLa cells using HADHA antibody. Western blot was performed from the immunoprecipitate using this antibody.
Western Blot: HADHA AntibodyBSA Free [NBP3-03743]

Western Blot: HADHA AntibodyBSA Free [NBP3-03743]

Western Blot: HADHA Antibody [NBP3-03743] - Western blot analysis of extracts of K-562 cells, using HADHA antibody (NBP3-03743) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: HADHA Antibody - BSA Free [NBP3-03743]

Immunocytochemistry/ Immunofluorescence: HADHA Antibody - BSA Free [NBP3-03743]

Immunocytochemistry/Immunofluorescence: HADHA Antibody [NBP3-03743] - Analysis of U2OS cells using HADHA antibody. Blue: DAPI for nuclear staining.
HADHA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HADHA Antibody - BSA Free [NBP3-03743] -

Immunocytochemistry/ Immunofluorescence: HADHA Antibody - BSA Free [NBP3-03743] - Immunofluorescence analysis of A-549 cells using [KO Validated] HADHA Rabbit pAb (A5346). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for HADHA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50-1:100

Western Blot

1:2000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HADHA

HADHA encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

Alternate Names

3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit, 3-oxoacyl-CoA thiolase, 78 kDa gastrin-binding protein, ECHA, gastrin-binding protein, GBP, HADH, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase(trifunctional protein), alpha subunit, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme Athiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit, LCEH, LCHAD, long-chain 2-enoyl-CoA hydratase, long-chain-3-hydroxyacyl-CoA dehydrogenase, MGC1728, mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit, mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alphasubunit, mitochondrial trifunctional enzyme, alpha subunit, mitochondrial trifunctional protein, alpha subunit, MTPATP-ALPHA, TP-alpha, trifunctional enzyme subunit alpha, mitochondrial

Gene Symbol

HADHA

Additional HADHA Products

Product Documents for HADHA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HADHA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HADHA Antibody - BSA Free

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Protocols

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