HAP1 Antibody (1B6) - BSA Free

Novus Biologicals | Catalog # NB110-74569

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B6

Format

BSA Free
View all HAP1 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from rat HAP1. [UniProt# P54256]

Specificity

This is specific for isoforms A and B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse HAP1 Antibody (1B6) - BSA Free (NB110-74569) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-HAP1 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HAP1 Antibody (1B6) - BSA Free

Western Blot: HAP1 Antibody (1B6) [NB110-74569]

Western Blot: HAP1 Antibody (1B6) [NB110-74569]

Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]

Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]

Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.
Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]

Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]

Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Western Blot: HAP1 Antibody (1B6) [NB110-74569]

Western Blot: HAP1 Antibody (1B6) [NB110-74569]

Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.

Applications for HAP1 Antibody (1B6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunohistochemistry

0.5-1.0 ug/ml

Immunohistochemistry-Frozen

0.5-1.0 ug/ml

Immunohistochemistry-Paraffin

0.5-1.0 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

0.5-1.0 ug/ml
Application Notes
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.

Reviewed Applications

Read 1 review rated 5 using NB110-74569 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HAP1

HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.

Alternate Names

HAP2huntingtin-associated protein 2, hHLP1, HIP5, HLP1, HLPHAP-1, huntingtin-associated protein 1, Neuroan 1

Gene Symbol

HAP1

Additional HAP1 Products

Product Documents for HAP1 Antibody (1B6) - BSA Free

Certificate of Analysis

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Product Specific Notices for HAP1 Antibody (1B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HAP1 Antibody (1B6) - BSA Free

Customer Reviews for HAP1 Antibody (1B6) - BSA Free (1)

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  • HAP1 Antibody (1B6)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse brain, PC12 cell lysate and Hela whole cell lysate
    Species: Rat, Mouse and Human
    Verified Customer | Posted 06/19/2018
    both endogenous isoforms visible in PC12 lysate (left lane is molecular weight marker)
    HAP1 Antibody (1B6) - BSA Free NB110-74569

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