HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14060

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-HDAC3-2D4

Format

Azide and BSA Free
View all Histone Deacetylase 3/HDAC3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human HDAC3 protein (Uniprot: O15379)

Localization

Nucleus, Nucleoplasm, Golgi apparatus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13809).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060]

Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060]

Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3 Antibody (PCRP-HDAC3-2D4).
HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060] -

Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060] -

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060] -

Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Applications for HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Gene Symbol

HDAC3

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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