HDAC3 Antibody

Novus Biologicals | Catalog # NB100-79798

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone Deacetylase 3/HDAC3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 375 and the C-terminus (residue 428) of human Histone Deacetylase 3 using the numbering given in Swiss-Prot entry O15379 (GeneID 8841).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC3 Antibody

Western Blot: HDAC3 Antibody [NB100-79798]

Western Blot: HDAC3 Antibody [NB100-79798]

Western Blot: HDAC3 Antibody [NB100-79798] - A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody NB100-79798 used at 0.02 mcg/ml for WB (A) and at 3 mcg/mg lysate for IP (B).
Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798]

Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798]

Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for HDAC3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Entrez Gene IDs

8841 (Human); 15183 (Mouse)

Gene Symbol

HDAC3

UniProt

O15379 (Human)

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody

Certificate of Analysis

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Product Specific Notices for HDAC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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