HDAC6 Antibody (OTI1B3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71531

Novus Biologicals
Discontinued Product
NBP2-71531 has been discontinued. View all Histone Deacetylase 6/HDAC6 products.

Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B3

Format

Azide and BSA Free
View all Histone Deacetylase 6/HDAC6 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

131.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC6 Antibody (OTI1B3) - Azide and BSA Free

Western Blot: HDAC6 Antibody (OTI1B3)Azide and BSA Free [NBP2-71531]

Western Blot: HDAC6 Antibody (OTI1B3)Azide and BSA Free [NBP2-71531]

Western Blot: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.
Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6.
Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - Staining of paraffin-embedded Human liver tissue using anti-HDAC6 mouse monoclonal antibody.
Flow Cytometry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Flow Cytometry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Flow Cytometry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HDAC6 antibody, and then analyzed by flow cytometry.
Western Blot: HDAC6 Antibody (OTI1B3)Azide and BSA Free [NBP2-71531]

Western Blot: HDAC6 Antibody (OTI1B3)Azide and BSA Free [NBP2-71531]

Western Blot: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.
Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - Staining of paraffin-embedded Human Kidney tissue using anti-HDAC6 mouse monoclonal antibody.
Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531]

Immunohistochemistry: HDAC6 Antibody (OTI1B3) - Azide and BSA Free [NBP2-71531] - Staining of paraffin-embedded Human pancreas tissue using anti-HDAC6 mouse monoclonal antibody.

Applications for HDAC6 Antibody (OTI1B3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone Deacetylase 6/HDAC6

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Long Name

Histone Deacetylase 6

Alternate Names

CPBHM, FLJ16239, HD6, HDAC6, JM21, PPP1R90

Gene Symbol

HDAC6

Additional Histone Deacetylase 6/HDAC6 Products

Product Documents for HDAC6 Antibody (OTI1B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC6 Antibody (OTI1B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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