HDAC7 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-16795
![Immunocytochemistry/ Immunofluorescence: HDAC7 Antibody [NBP2-16795]](https://resources.rndsystems.com/images/products/HDAC7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16795-img0004.jpg "Immunocytochemistry/ Immunofluorescence: HDAC7 Antibody [NBP2-16795]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (93%), Mouse (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human HDAC7. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HDAC7 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: HDAC7 Antibody [NBP2-16795]
Immunocytochemistry/Immunofluorescence: HDAC7 Antibody [NBP2-16795] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.![Immunohistochemistry-Paraffin: HDAC7 Antibody [NBP2-16795]](https://resources.rndsystems.com/images/products/HDAC7-Antibody-Immunohistochemistry-Paraffin-NBP2-16795-img0002.jpg "Immunohistochemistry-Paraffin: HDAC7 Antibody [NBP2-16795]")
Immunohistochemistry-Paraffin: HDAC7 Antibody [NBP2-16795]
Immunohistochemistry-Paraffin: HDAC7 Antibody [NBP2-16795] - Human colon carcinoma, using HDAC7 antibody at 1:250 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min![Immunoprecipitation: HDAC7 Antibody [NBP2-16795]](https://resources.rndsystems.com/images/products/HDAC7-Antibody-Immunoprecipitation-NBP2-16795-img0003.jpg "Immunoprecipitation: HDAC7 Antibody [NBP2-16795]")
Immunoprecipitation: HDAC7 Antibody [NBP2-16795]
Immunoprecipitation: HDAC7 Antibody [NBP2-16795] - 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 ug of HDAC7 antibody 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Western Blot: HDAC7 Antibody [NBP2-16795] -
Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC7 antibody [C2C3], C-term (NBP2-16795) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for HDAC7 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:500-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HDAC7
Long Name
Histone deacetylase 7
Alternate Names
HD7, HD7a, HDAC7A
Gene Symbol
HDAC7
UniProt
Additional HDAC7 Products
Product Documents for HDAC7 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HDAC7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for HDAC7 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for HDAC7 Antibody - BSA Free
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Q: Do the following antibodies cross-react with any other the HDAC's? 1) NBP2-22152 and 2) NBP2-16795
A: We have not actually tested these for cross reactivity. I have looked at homology between he HDAC5/HDAC7 proteins and they are generally only about 60% homologous. As polyclonal antibodies they may cross react slightly.
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